Investigating Lysosomal Dysfunction in Ageing and Neurodegeneration
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UK
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University of Dundee
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On Campus (Full Time)
3 years-
PhD Studentship
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Unspecified
On Campus (Full Time) Position Details (PhD Program)
In this Investigating Lysosomal Dysfunction in Ageing and Neurodegeneration project at University of Dundee, the plan is to characterise the lysosomal content in terms of proteins, lipids and metabolites in cells derived from peripheral blood from people with PD /AD and healthy controls.
This Investigating Lysosomal Dysfunction in Ageing and Neurodegeneration Investigating Lysosomal Dysfunction in Ageing and Neurodegeneration is offered at University of Dundee University of Dundee .
Context
Lysosomes are key cellular organelles that degrade and recycle macromolecules, act as signalling hubs and are implicated in many diseases including Parkinson’s disease (PD) and Alzheimer’s dementia (AD) where old age is a major risk factor. Studying lysosomal biology and dysfunction in human diseases has been challenging because lysosomes represent less than 3% of the total cell volume, such that changes in lysosomal content are hardly detected using traditional whole tissue/cell measurements.
We have refined a recently developed approach for the rapid immunoaffinity purification (IP) of intact lysosomes using a recombinant tag on the external lysosomal transmembrane protein for use
- in the endogenous system
- use in human peripheral blood mononuclear cells