PhD Astrocyte-Synapse Topology, Regulation Adaptability through Light, X-ray and Electron Microscopy

The European Synchrotron, the ESRF, is an international research centre based in Grenoble, France.

Through its innovative engineering, pioneering scientific vision and a strong commitment from its 700 staff members, the ESRF is recognised as one of the top research facilities worldwide. Its particle accelerator produces intense X-ray beams that are used by thousands of scientists each year for experiments in diverse fields such as biology, medicine, environmental sciences, cultural heritage, materials science, and physics.

Supported by 19 countries, the ESRF is an equal opportunity employer and encourages diversity.

Thesis subject: Astrocyte-Synapse Topology, Regulation and Adaptability through Light, X-ray and Electron Microscopy (Cryo-ASTRALXEM) The Cryo-ASTRALXEM PhD project aims to decipher the nanoscale organization of astrocyte-synapse interfaces using a multimodal cryogenic imaging workflow. You will combine cryogenic X-ray holographic nanotomography, visible light microscopy, cryogenic X-ray fluorescence, and cryo-electron tomography to bridge scales from whole astrocytes within their native context all the way down to molecular resolution.

You will develop and optimize molecular probes and labelling strategies, including immunolabelling, nanobodies, and genetically encoded tags (APEX/Halo), that are compatible with high-pressure freezing, vitrification, and cryogenic X-ray/electron imaging.

The project also includes establishing multimodal correlation workflows and collaborating with machine learning specialists to implement advanced computational analysis, including ML-based segmentation and 3D quantitative mapping.

Overall, the project aims to reveal how astrocytic microdomains are structured and regulated, and how they adapt to their microenvironment.

You will join the X-ray nanoscale neuroimaging unit at ESRF and work closely with Dr. Sylvain Bohic from INSERM and UGA.

Further information may be obtained from:

Lucas BENOIT (tel.: +33 (0)4 76 88 24 88, email: [emailprotected]).

- Degree allowing enrolment for a PhD (such as MSc, Master 2 de Recherche, Laurea or equivalent) in Neurosciences or Biology. A degree in biophysics, chemistry or physics could also be compatible.

- The candidate must have obtained and provide proof of a diploma at the time of application. The European Commission/MSCA may consider a student eligible provided that the university issues an official certificate confirming that the degree has been completed. The diploma will have to be provided at a later stage and is a mandatory requirement for enrolment in a doctoral school.

- Experience with data analysis, histology and cryogenic sample preparation for electron microscopy or X-ray would be an important asset

- Experience in viral production, vector production, as well as their handling for the generation of biological probes would be a major asset.

- Previous experience with surgery would be an advantage.

- Ease and versatility in the use of computer systems are highly recommended.

- Proficiency in English (A proof of upper-intermediate B2 level must be included in the application. Applicants originating from native-English-speaking countries can apply without the need for proof of level. An official degree conducted in English will be also accepted as a proof). If the applicant is unable to provide proof, an English test (free of charge) will be requested.

- Compliance with the Marie Sklodowska-Curie mobility rule: candidates may not have resided or carried out their main activity (work, studies, etc.) in the host institute's country for more than twelve months in the three years immediately before the application deadline (31/03/2026).

- All researchers recruited must be doctoral candidates, i.e. not already working towards or in possession of a doctoral degree at the date of the recruitment.

You shall be enrolled in Grenoble-Alpes University (UGA) Doctoral School of Chemistry and Life Sciences (EDCSV).

Contract of two years, renewable for one year

Do you recognize yourself in this description? Apply now for your next professional adventure!

What we offer:

1. Join an innovative international research institute, with a workforce from 38 different countries

2. Collaborate with global experts to advance science and address societal challenges

3. Come and live in a vibrant city, in the heart of the Alps, and Europe's Green Capital 2022

4. Enjoy a workplace designed to support your quality of life

5. Benefit from ourcompetitive compensation and allowances package, including financial support for your relocation to Grenoble

For further information on employment terms and conditions, please refer to https://www.esrf.fr/home/Jobs/what-we-offer.html

The ESRF is an equal opportunity employer and encourages applications from disabled persons.